CUZ DAVID FELDMAN REALLY IS A BIG DEAL

It’s a big deal because Feldman basically removed a really stupid trade-off biologists were stuck with:

Before:

• You could screen

tons

• Or you could get gorgeous, information-dense images… but only for a tiny panel of perturbations because arrayed imaging is slow, expensive, and batch-gross.

Optical pooled screening is “have your cake and eat the 10⁶-perturbation library too.”

1. What optical pooled screening actually is

Very roughly:

1. You do a pooled CRISPR / perturbation screen:

   A big lentiviral library of perturbations (sgRNAs, barcodes, etc.) gets delivered into a large population of cells.