• Engineering cell lines with reporters
• Optimizing reporters and treating engineered cells with a variety of conditions (long term)
• Making a good robust protocol for imaging and imaging analysis (short term)
  • focus on getting good images for data analysis
• Determine what factors changes cell morphology and redox status in endometrial cancer cells
• Design UPU for project
  • in Benchling account
  • Outline questions I’ll be asking
  • outline techniques
• Initial phase:
  • Thaw cells and provide own samples to test for microscope training
• Design matrix of plus/minus treatments
• Using CellProfiler to do masking and quantitative analysis of images
• Create outlook calendar and send more info regarding deadlines and assignments for BIOL 395 to Karl and Tig
• Reading scientific literature
• Mondays, Wednesday, Fridays are experiment days
• Tuesdays and Thursdays are small tasks (lab notebook updated); desk work days