Calculator

Source file: (Common) Calculator.xlsx

Formula behavior

  1. Notion simple tables do not execute Excel-style cell formulas such as =SUM(...) or =C16*C17*C18.
  2. Formula cells are preserved below as formula text so the original calculation logic is visible.
  3. To make the calculator actually interactive like Excel, it must remain as an .xlsx/Google Sheet, or be rebuilt as Notion databases with formula properties for each calculator.

Index

No. Name
1 PCR
2 Restriction enzyme cutting
3 T4 ligation
4 DNA ligation
5 용액 희석 계산기
6 Cas9 purification buffer
7 몰농도 계산기
8 In vitro cleavage
9 sgRNA Extension PCR
10 In vitro transcription
11 Gibson assembly
12 In-fusion cloning
13 Coomassie R250 staining solution
14 Destaining solution

1. PCR

Component Volume Unit
dNTP 4 uL
10X buffer 5 uL
Pyrobest/Taq 1 uL
Template 1 uL
Primer F+R (10pmol) 4 uL
Pure H2O =C12-C10-C9-C8-C7-C6 uL
Total 50 uL

2. Restriction enzyme cutting

Component Volume Unit
Plasmid 35 uL
Restriction enzyme #1 5 uL
Restriction enzyme #2 0 uL
10X buffer =G13/10 uL
Pure H2O =G13-G9-G8-G7-G6 uL
반응시간 2-3hr or O/N
반응온도 37 C
Total 50 uL

3. T4 ligation

Component Volume Unit
Linear vector DNA (BB) 4 uL
Insert DNA (i) 1 uL
10X T4 buffer 2 uL
T4 DNA ligase 1 uL
Pure H2O =17-K6-K7 uL
반응시간 30 min
반응온도 22 C
Total 20 uL

4. DNA ligation (1:3)

Input Volume / value Unit
Insert 1440 bp
Backbone 4370 bp
BB DNA mass =36*7 ng
Require insert DNA mass =O8*(O6/O7)*3 ng

4. DNA ligation (1:5)

Input Volume / value Unit
Insert 60 bp
Backbone 13958 bp
BB DNA mass 100 ng
Require insert DNA mass =O14*(O12/O13)*5 ng

4. DNA ligation (1:7)

Input Volume / value Unit
Insert 800 bp
Backbone 12385 bp
BB DNA mass 1890 ng
Require insert DNA mass =O20*(O18/O19)*7 ng