Lentivirus infection to normal cell (Transduction)

Day 1

1. Seed the cells in 100mm dish plate.
2. 다음날 plate의 70%정도 자랄 수 있도록 seeding (약 2.5 x 10^6 cells/10mL)

Day 2

1. Remove media - 1mL(1X) or 100uL(10X).
2. Add 1mL(1X) of the virus solution to each well.
3. Alternatively, add 100uL(10X) of the virus solution to each well.
4. Add 8 ug/mL of polybrene.
5. Incubate at 37C for 24 hr.

Day 3

1. Change media to Growth Media with selection antibiotics, for example Puromycin 2-4 ug/mL, and incubate for 24 hr.
2. Change Selection Media every 24 hr for 3-5 days to remove dead cells.
3. After 5 days, cells should have reached 100% confluency.