Cell protocols

  1. Cell Fractionation
  2. FACS for Analysis
  3. FACS for Sorting
  4. Lentivirus infection to PBMC (Transduction)
  5. Lentivirus infection to normal cell (Transduction)
  6. RNA Prep - TRIzol
  7. SYBR qPCR
  8. Taqman qPCR
  9. Transduction volume calculator
  10. Transfection using PEI-pro - Lentivirus packaging
  11. Transfection using PEI-pro - Lentivirus packaging calculator
  12. cDNA synthesis
  13. shRNA lentivirus production

Protocol links will be populated after upload.

Protocol | Cell Fractionation

Protocol | FACS for Analysis

Protocol | FACS for Sorting

Protocol | Lentivirus infection to PBMC (Transduction)